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Updated: Aug 22, 2025

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Modeling Human Gonad Development in Organoids.

Marina V Pryzhkova1,2, Romina Boers3,4, Philip W Jordan5,6

  • 1Department of Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, 615 N. Wolfe St., Baltimore, MD, 21205, USA. marina.pryzhkova.ctr@usuhs.edu.

Tissue Engineering and Regenerative Medicine
|November 9, 2022
PubMed
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This summary is machine-generated.

Researchers developed a novel 3D human pluripotent stem cell (hPSC) organoid system to model early human gonad development. This breakthrough advances the study of infertility and sex development disorders.

Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Reproductive Medicine

Background:

  • Studying human reproductive development is challenging due to limitations of animal models and human fetal tissues.
  • Three-dimensional (3D) organoid technology using human pluripotent stem cells (hPSCs) offers a promising in vitro model for tissue development.
  • A system for modeling human gonad development has been lacking, hindering research into infertility causes.

Purpose of the Study:

  • To establish a functional in vitro model for early human gonad development using hPSC-derived organoids.
  • To investigate the self-organizing and differentiation capabilities of hPSCs in mimicking embryonic gonad development.
  • To create a system for studying the causes of infertility and sex development disorders.

Main Methods:

Keywords:
Gonad developmentHuman pluripotent stem cellsMesonephrosMini-spin bioreactorTestis organoid model

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  • Utilized 3D hPSC organoid culture within mini-spin bioreactors.
  • Leveraged intrinsic self-organization and differentiation of stem cells.
  • Explored the mimicry of human embryonic and fetal gonad development.

Main Results:

  • Developed a simple, bioreactor-based organoid system for modeling early human gonad development.
  • Male hPSC-derived organoids successfully recapitulated embryonic gonad trajectory.
  • Organoids differentiated into multipotent progenitors, specializing into testicular supporting and interstitial cells, with characteristic tissue organization and functional marker expression.

Conclusions:

  • The developed organoid system enables detailed studies of human gonad and germ cell development.
  • This technology can advance understanding of sex development disorders and infertility.
  • Human gonad organoid technology holds potential for regenerative medicine applications.