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A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells
Christopher Rainville1, David E Sterner1, Kumar Suresh2
1Progenra Inc, Malvern, PA, USA.
Abstract:
SARS-CoV-2 protease Nsp3 is a therapeutic target for developing anti-SARS-CoV-2 drugs. Nsp3 is a large multi-spanning membrane protein, and its characterization in vitro has been challenging. Here we describe an in vitro assay to characterize the biochemical activity of full-length Nsp3 isolated from cells. The assay can be used to evaluate Nsp3 inhibitors.
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