Related Experiment Video
Updated: Aug 22, 2025

A High-Yield Streptomyces Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
Published on: September 10, 2021
Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens
Binbin Gu1, Bernd Goldfuss2, Jeroen S Dickschat1
1Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121, Bonn, Germany.
Abstract:
The sesterviolene synthase from Streptomyces violens was identified and represents the second known sesterterpene synthase from bacteria. Isotopic labelling experiments in conjunction with DFT calculations were performed that provided detailed insight into its complex cyclisation mechanism. Enzyme engineering through site-directed mutagenesis gave access to a high-yielding enzyme variant that provided six additional minor products and the main product in sufficient quantities to study its chemistry.
More Related Videos
07:59A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
09:31PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Published on: September 26, 2020
Related Concept Videos
ATP Synthase: Mechanism
E2 Reaction: Kinetics and Mechanism
Biosynthesis in Bacteria
E1 Reaction: Kinetics and Mechanism
ATP Synthase: Structure
Introduction to Mechanisms of Enzyme Catalysis