A Molecular Lateral Flow Assay for SARS-CoV-2 Quantitative Detection.

Area of Science:

• Biotechnology
• Molecular Diagnostics
• Nanotechnology

Background:

• The SARS-CoV-2 pandemic highlighted the need for rapid and accurate diagnostic tools.
• Existing COVID-19 detection methods vary in performance, sampling, and analyte types.
• Knowledge gained from developing new assays can prepare for future infectious outbreaks.

Purpose of the Study:

• To develop a novel gold-nanoparticle-based nucleic acid lateral flow assay for SARS-CoV-2 detection.
• To enable rapid, visual, and quantitative detection of the virus.
• To create a cost-effective and accurate diagnostic tool for current and future pandemics.

Main Methods:

• Utilized a DNA internal standard for quantification.
• Employed reverse transcription-polymerase chain reaction (RT-PCR).
• Detected biotinylated PCR products using gold nanoparticles conjugated to anti-biotin antibodies on a dual lateral flow assay.

Main Results:

• Achieved rapid, naked-eye detection of SARS-CoV-2.
• Enabled simultaneous quantification of the virus in nasopharyngeal swabs.
• Demonstrated high specificity, detectability, and repeatability of the assay.

Conclusions:

• The developed molecular strip test is a simple, cost-effective, and accurate rapid diagnostic tool.
• This assay shows promise for controlling the current pandemic and managing future infectious outbreaks.
• Gold nanoparticle technology offers a versatile platform for molecular diagnostics.