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Related Experiment Video

Updated: Aug 22, 2025

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses
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Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19.

Patarajarin Akarapipad1, Elizabeth Bertelson1, Alexander Pessell1

  • 1Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.

Biosensors
|November 10, 2022
PubMed
Summary

Multiplex nucleic acid diagnostic tests are crucial for detecting SARS-CoV-2 variants and other respiratory viruses. This review covers current multiplex assays, their performance, and future directions for point-of-care diagnostics.

Keywords:
COVID-19SARS-CoV-2multiplexnucleic acid amplification testingpoint-of-carevirus

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Area of Science:

  • Molecular diagnostics
  • Virology
  • Infectious disease testing

Background:

  • The COVID-19 pandemic highlighted the need for rapid and accurate diagnostic testing for SARS-CoV-2.
  • Emerging variants and other respiratory viruses necessitate multiplex detection beyond single-target assays.
  • Sequencing is often too slow and costly for routine diagnostic use.

Purpose of the Study:

  • To review advances in multiplex nucleic acid-based virus diagnostic testing.
  • To provide a comprehensive overview of current multiplex assays, targets, and detection methods.
  • To discuss challenges and future perspectives in multiplex diagnostic test development.

Main Methods:

  • Literature review of frequent nucleic acid targets for multiplex virus diagnostic tests.
  • Comprehensive overview of multiplex assays, including detection reactions and readout modalities.
  • Analysis of assay performance, advantages, disadvantages, and point-of-care platforms.

Main Results:

  • Identified frequent nucleic acid targets for multiplex virus detection.
  • Detailed review of various multiplex assay technologies and their characteristics.
  • Highlighted point-of-care adaptable platforms and discussed their performance metrics.

Conclusions:

  • Multiplex nucleic acid tests are essential for comprehensive respiratory virus surveillance.
  • Current multiplex assays offer diverse approaches with varying performance profiles.
  • Further research is needed to address technical challenges and enhance point-of-care capabilities.