A Mediated Enzymatic Electrochemical Sensor Using Paper-Based Laser-Induced Graphene.

Area of Science:

• Materials Science
• Biomedical Engineering
• Analytical Chemistry

Background:

• Laser-induced graphene (LIG) is versatile for sensing applications.
• Paper-based LIG (PaperLIG) offers potential for low-cost, flexible biosensors.
• PaperLIG fabrication differs from polyimide-based LIG.

Purpose of the Study:

• To fabricate a highly conductive PaperLIG.
• To develop a PaperLIG-based glucose biosensor.
• To evaluate the biosensor's performance and potential for diabetes management.

Main Methods:

• Two-step fabrication of PaperLIG.
• Immobilization of glucose oxidase, aminoferrocene, and Nafion on PaperLIG.
• Amperometric measurement of glucose at -90 mV.

Main Results:

• Characterization confirmed PaperLIG suitability for biosensors.
• The biosensor achieved a limit of detection of 50-75 µM and a linear range of 100 µM to 3 mM.
• Nafion concentration effects were investigated; saliva interferences were negligible.

Conclusions:

• A simple, low-cost PaperLIG glucose biosensor was successfully developed.
• The biosensor demonstrates good performance for glucose detection in saliva.
• This PaperLIG biosensor holds promise for future diabetes management.