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ODDC and MULTIGRAPH: programs for spectrophotometer data collection and manipulation using a microcomputer.

S M Ross, A T Black, C P Reynolds

    Computer Methods and Programs in Biomedicine
    |April 1, 1986
    PubMed
    Summary
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    This software package collects, graphs, and prints spectrophotometer data using an Apple II computer and ADALAB card. It is ideal for column chromatography due to its peak sensing and data annotation capabilities.

    Area of Science:

    • Analytical Chemistry
    • Computer Science

    Background:

    • Spectrophotometry is a crucial technique in analytical chemistry.
    • Efficient data acquisition and analysis are vital for chromatographic methods.
    • Limited user-friendly software existed for integrating spectrophotometers with early microcomputers.

    Purpose of the Study:

    • To develop a comprehensive software package for spectrophotometer data handling.
    • To enhance the utility of the Apple II microcomputer for scientific data acquisition.
    • To provide peak sensing and data annotation features for column chromatography applications.

    Main Methods:

    • Developed a software package utilizing interpreted and compiled BASIC programs with machine language subroutines.
    • Interfaced a spectrophotometer with an Apple II microcomputer via an ADALAB analog-to-digital converter card.

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  • Implemented a menu-driven system for controlling data collection, storage, recall, graphing, and printing.

    • Main Results:

    • The software package efficiently collects, stores, graphs, and prints spectrophotometer data.
    • The system supports storage of up to 12,600 data points (7 hours of data).
    • Integrated peak sensing and time-point annotation capabilities were achieved.

    Conclusions:

    • The developed software package provides a robust solution for spectrophotometer data management on an Apple II system.
    • The software's features, including peak sensing, make it highly suitable for column chromatography.
    • This integration enhances the capabilities of microcomputers in scientific research settings.