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Updated: Aug 22, 2025

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Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
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Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells
Angela R Armiento1,2, Yann D Ladner1,3, Elena Della Bella1
1AO Research Institute Davos, Davos Platz, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|November 10, 2022
Abstract:
Bone marrow-derived mesenchymal stromal cells (BM-MSC) are widely studied in the field of cartilage regeneration due to their capacity to differentiate into chondrocytes under specific in vitro culture conditions. This chapter describes the isolation of MSC from bone marrow aspirate, their expansion in monolayer, and the chondrogenic differentiation in pellet culture.

