Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Towards a REASSURED reality: A less-is-more electronic design strategy for self-powered glucose test.

Biosensors & bioelectronics·2023

Same author

Unraveling the Most Relevant Features for the Design of Iridium Mixed Oxides with High Activity and Durability for the Oxygen Evolution Reaction in Acidic Media.

JACS Au·2023

Same author

Active and durable R<sub>2</sub>MnRuO<sub>7</sub> pyrochlores with low Ru content for acidic oxygen evolution.

Nature communications·2023

Same author

Nanoceria dissolution at acidic pH by breaking off the catalytic loop.

Nanoscale·2022

Same author

A plant-like battery: a biodegradable power source ecodesigned for precision agriculture.

Energy & environmental science·2022

Same author

Microfluidics for Electrochemical Energy Conversion.

Chemical reviews·2022

Search research articles

Related Experiment Video

Updated: Aug 22, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Immuno-battery: A single use self-powered immunosensor for REASSURED diagnostics.

Dmitry Galyamin¹, Susana Liébana¹, Juan Pablo Esquivel²

¹Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), C/ dels Til⋅lers, Campus UAB, 08193, Bellaterra, Barcelona, Spain.

Biosensors & Bioelectronics

|November 10, 2022

Summary

This study introduces a novel self-powered paper-based immunosensor, the Immuno-Battery, which generates its own energy to detect biomarkers like C-reactive protein (CRP). This sustainable device offers a promising, affordable approach for future digital diagnostics.

Keywords:

Diagnostics Immunoassay REASSURED Self-powered sensor ePAD

More Related Videos

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Related Experiment Videos

Last Updated: Aug 22, 2025

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Area of Science:

Biomedical Engineering
Electrochemistry
Biosensors

Background:

Conventional diagnostic devices often require external power sources, limiting their accessibility and affordability.
Paper-based electrochemical immunoassays are promising for point-of-care diagnostics but can be limited by power requirements.

Purpose of the Study:

To develop a novel, self-powered paper-based immunosensor for biomarker detection.
To demonstrate the feasibility of using an "Immuno-Battery" for energy generation during the sensing process.
To evaluate the performance and sensitivity of the self-powered immunosensor for C-reactive protein (CRP) detection.

Main Methods:

Development of a paper-based immunosensor utilizing magnesium as an anode and HRP-labeled antibodies as a cathodic catalyst.
Integration of self-powering capabilities, generating energy from the biomarker-induced reaction.
Testing the device under resistive load to measure operational voltage and power density.
Assessing the limit of detection (LOD) for CRP in artificial samples and comparing it to existing methods.

Main Results:

The Immuno-Battery achieved operational voltages above 1.55 V and power densities ranging from 40 to 571 μW cm⁻².
The self-powered operation did not compromise sensitivity, with a demonstrated LOD of 20 ± 2 ng mL⁻¹ for protein detection.
A specific LOD of 269 ± 39 ng mL⁻¹ was achieved for C-reactive protein (CRP), comparable to commercial kits.

Conclusions:

A novel self-powered paper-based immunosensor (Immuno-Battery) has been successfully developed and demonstrated.
The device generates sufficient power for potential integration with electronic readout circuits.
This technology paves the way for sustainable, affordable, and accessible digital diagnostic devices.