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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

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Bioactive Components and Health Potential of Endophytic Micro-Fungal Diversity in Medicinal Plants

Sundaram Muthukrishnan1, Paranivasakam Prakathi1, Thangavel Sivakumar2

  • 1Department of Biotechnology, Ayya Nadar Janaki Ammal College, Sivakasi 626124, Tamil Nadu, India.

Antibiotics (Basel, Switzerland)
|November 11, 2022
PubMed

Insights

Endophytic fungi from medicinal plants yield bioactive compounds with antibacterial and antioxidant properties. Promising compounds like Brallobarbital show potential for developing new anticancer drugs against lung carcinoma.

Area of Science:

  • Microbiology
  • Pharmacology
  • Natural Products Chemistry

Background:

  • Endophytic fungi within medicinal plants are a rich source of pharmacologically active compounds.
  • Isolation of specific endophytic fungi, including Graminicolous helminthosporium, Bipolaris australiensis, and Cladosporium cladosporioides, from diverse medicinal plants.

Purpose of the Study:

  • To identify and characterize bioactive metabolites from endophytic fungi.
  • To evaluate the antibacterial and antioxidant activities of fungal extracts.
  • To investigate the anticancer potential of identified compounds against non-small cell lung carcinoma.

Main Methods:

  • Gas Chromatography-Mass Spectrometry (GC-MS) for metabolite profiling.
  • Phytochemical analysis for compound identification (flavonoids, phenols, etc.).
  • Agar-well diffusion and DPPH, H2O2, nitric oxide scavenging assays for bioactivity screening.
  • Molecular docking against EGFR for anticancer potential assessment.

Main Results:

  • GC-MS revealed diverse bioactive metabolites including Brallobarbital and 5 methyl 2 phenylindolizine.
  • Endophytic extracts demonstrated significant antibacterial and antioxidant activities.
  • Molecular docking indicated Brallobarbital and 5 methyl 2 phenylindolizine as potential EGFR inhibitors for lung cancer.

Conclusions:

  • Isolated endophytic fungi are valuable sources of novel bioactive compounds.
  • These compounds exhibit promising therapeutic potential for antibacterial, antioxidant, and anticancer applications.
  • Natural product drug development from endophytic fungi offers a sustainable alternative to synthetic drugs.
Keywords:
FTIRGC-MSantibacterialantioxidantbioactive compoundsentophytic fungimolecular docking

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