Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.0K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.0K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Embryonic Origins of Cancer: Insights from Double Homeobox 4 Regulation.

Biomolecules·2025
Same author

CRISPR Technology Acts as a Dual-Purpose Tool in Pig Breeding: Enhancing Both Agricultural Productivity and Biomedical Applications.

Biomolecules·2024
Same author

Essential roles of the nucleolus during early embryonic development: a regulatory hub for chromatin organization.

Open biology·2024
Same author

Porcine oviductal extracellular vesicles facilitate early embryonic development via relief of endoplasmic reticulum stress.

Cell biology international·2021
Same author

Functions and Regulation of Endogenous Retrovirus Elements during Zygotic Genome Activation: Implications for Improving Somatic Cell Nuclear Transfer Efficiency.

Biomolecules·2021
Same author

Extracellular Vesicles Function as Bioactive Molecular Transmitters in the Mammalian Oviduct: An Inspiration for Optimizing in Vitro Culture Systems and Improving Delivery of Exogenous Nucleic Acids during Preimplantation Embryonic Development.

International journal of molecular sciences·2020

Related Experiment Video

Updated: Aug 22, 2025

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

12.7K

2-Cell-like Cells: An Avenue for Improving SCNT Efficiency.

Bo Fu1,2, Hong Ma1,2, Di Liu1,2

  • 1Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China.

Biomolecules
|November 11, 2022
PubMed
Summary

Totipotency, the ability of a cell to differentiate into any cell type, can be transiently restored in embryonic stem cells (ESCs) and may improve somatic cell nuclear transfer (SCNT) efficiency.

Keywords:
2-cell-like cellspreimplantation embryosomatic cell nuclear transfertotipotency

More Related Videos

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

14.3K
Generation of Mice Derived from Induced Pluripotent Stem Cells
11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

21.7K

Related Experiment Videos

Last Updated: Aug 22, 2025

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
09:52

Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Published on: April 26, 2018

12.7K
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
13:36

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT

Published on: September 30, 2010

14.3K
Generation of Mice Derived from Induced Pluripotent Stem Cells
11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

21.7K

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Stem Cell Biology

Background:

  • Zygote genome reorganization establishes totipotency, which then declines.
  • Embryonic stem cells (ESCs) can transiently regain totipotency.
  • Somatic cell nuclear transfer (SCNT) often shows deficient totipotency establishment in cloned embryos.

Purpose of the Study:

  • To review strategies for inducing 2-cell-like cells (2CLCs) in cloned embryos.
  • To explore the potential of 2CLCs for improving SCNT efficiency.
  • To investigate pathways for establishing totipotency in cloned embryos.

Main Methods:

  • Review of existing literature on 2CLC induction and SCNT.
  • Analysis of transcriptome and chromatin features of 2CLCs.
  • Hypothesizing pathways for totipotency establishment in cloned embryos.

Main Results:

  • 2CLCs exhibit transcriptome and chromatin features similar to 2-cell-stage embryos.
  • Strategies to induce 2CLCs are being investigated in cloned embryo development.
  • A potential pathway for cloned embryos to establish totipotency is proposed.

Conclusions:

  • 2CLCs serve as a valuable in vitro model for studying totipotency.
  • Inducing 2CLCs may offer new avenues to enhance SCNT efficiency.
  • Further research into 2CLC induction could advance reproductive technologies.