A fixation-decalcification procedure for bone biopsies
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Updated: Aug 2, 2026

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Undecalcified Bone Preparation for Histology, Histomorphometry and Fluorochrome Analysis
Published on: January 8, 2010
Histopathology
|September 1, 1978
Abstract:
A procedure for the simultaneous fixation and decalcification of bone biopsies is described. Mild acidic fixatives (such as Bouin's fluid) are effective decalcifying agents when made calcium-free by a continuous circulation through a column of anion-binding ion exchange resin. The procedure has been found relatively fast, safe, economic and to provide excellent histology of biopsies containing cancellous bone.

