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Are Antisense Long Non-Coding RNA Related to COVID-19?
Eman Ae Badr1, Ibrahim Eltantawy El Sayed2, Mohanad Kareem Razak Gabber2
1Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Menoufia University, Shebeen El-Kom 32511, Egypt.
Biomedicines
|November 11, 2022
Summary
Antisense long non-coding RNAs (lncRNAs) show altered expression in COVID-19 patients, correlating with disease severity. These RNA changes may play a role in the pathogenesis of coronavirus disease 2019.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Immune system gene expression is tightly regulated to fight pathogens.
- Ferroptosis, an iron-driven cell death, enhances T cell function during infection.
- RNA regulation is critical for controlling gene expression.
Purpose of the Study:
- To investigate the expression levels of specific antisense long non-coding RNAs (A2M-AS1, DBH-AS1, FLVCR1-DT, NCBP2AS2-1) and FLVCR1 in COVID-19 patients.
- To determine the relationship between the expression of these molecules and COVID-19 disease severity.
Main Methods:
- Quantitative reverse transcription PCR (RT-PCR) was used to measure the expression levels of selected antisense long non-coding RNAs and FLVCR1.
- COVID-19 patients and age/gender-matched healthy controls were recruited for the study.
Main Results:
- Decreased expression of A2M-AS1 and FLVCR1 was observed in COVID-19 patients.
- Increased expression of DBH-AS1, FLVCR1-DT, and NCBP2AS2 was noted.
- FLVCR1-DT and NCBP2AS2 positively correlated with interleukin-6 (IL-6).
- DBH-AS1 and FLVCR1-DT were associated with mortality, complications, and mechanical ventilation.
- Negative correlations were found between A2M-AS1 and NCBP2AS2-1, and between FLVCR1 and FLVCR1-DT.
Conclusions:
- Antisense long non-coding RNA expression is deregulated in COVID-19 patients.
- The altered expression levels correlate with COVID-19 severity.
- These findings suggest a potential role for antisense lncRNAs in the pathogenesis of COVID-19.
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