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RTCB Complex Regulates Stress-Induced tRNA Cleavage.

Yasutoshi Akiyama1, Yoshika Takenaka1, Tomoko Kasahara2

  • 1Laboratory of Oncology, Pharmacy Practice and Sciences, Tohoku University Graduate School of Pharmaceutical Sciences, Sendai 980-8578, Japan.

International Journal of Molecular Sciences
|November 11, 2022
PubMed
Summary
This summary is machine-generated.

RTCB ligase complex (RTCB-LC) negatively regulates stress-induced tRNA-derived RNAs (tiRNAs). RTCB-LC inhibition boosts tiRNA production, aiding cellular stress response.

Keywords:
RTCB ligase complexangiogeninoxidative stresstRNAtiRNAs

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Area of Science:

  • Molecular Biology
  • RNA Biology
  • Cellular Stress Response

Background:

  • Transfer RNAs (tRNAs) are cleaved into tRNA-derived RNAs (tiRNAs) under stress.
  • tiRNAs protect cells by inhibiting translation and apoptosis.
  • Regulation of tiRNA production is crucial for cellular stress adaptation.

Purpose of the Study:

  • To investigate the role of RTCB ligase complex (RTCB-LC) in regulating stress-induced tiRNA production.
  • To elucidate the mechanism by which RTCB-LC influences tiRNA levels.
  • To understand the contribution of RTCB-LC to cellular stress response.

Main Methods:

  • Knockdown of RTCB gene expression.
  • Analysis of tiRNA production under various stress conditions.
  • In vitro repair of tiRNAs using purified RtcB.
  • Investigating the effect of oxidative stress (H2O2) on tiRNA production.

Main Results:

  • RTCB knockdown significantly increased stress-induced tiRNA production.
  • RtcB demonstrated the ability to repair tiRNAs into full-length tRNAs in vitro.
  • Oxidative stress, while not inducing tiRNAs alone, boosted their production when stress-responsive RNases were active.
  • RTCB-LC was identified as a negative regulator and a boosting mechanism for tiRNA production.

Conclusions:

  • RTCB-LC negatively regulates the production of stress-induced tiRNAs.
  • RTCB-LC plays a role in generating full-length tRNAs from tiRNAs.
  • The RTCB-LC-mediated boosting mechanism contributes to cellular stress adaptation by modulating tiRNA levels.