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Combined Therapy of Probiotic Microcapsules and Bomidin in Vibrio parahaemolyticus-Infected Rats
Ting Zhou1, Mengting Liu1, Jialiang Pan1
1International Joint Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Background:
With the discovery of more and more drug-resistant bacterial strains, there is an urgent need for safer and more effective alternative treatments. In this study, antibacterial peptides and probiotic microcapsules were combined to treat gastrointestinal inflammation caused by Vibrio parahaemolyticus infection.
Methods:
To improve the stability of probiotics in the gastrointestinal tract, two types of mixed natural anionic polysaccharides and chitosan were used as carriers to embed the probiotics. Taking Lacticaseibacillus casei CGMCC1.8727 microcapsules with good performance as the research object, the in vitro characteristics of the microcapsules were studied via acid resistance test and intestinal release test. The microcapsules were then tested for in vivo treatment in combination with the antibacterial peptide, bomidin, and the therapeutic effects were compared among microencapsulated probiotics, free probiotics, and probiotics in combination with bomidin.
Results:
Microencapsulation was successfully manufactured under suitable processing parameters, with the product particle size being 2.04 ± 0.2743 mm. Compared with free probiotics, microencapsulation significantly improved the activity and preservation stability of the probiotics under simulated gastrointestinal conditions. Microencapsulated probiotics showed better therapeutic effects than free probiotics in vivo. Microcapsules combined with antimicrobial peptides accelerated the elimination of bacteria in vivo. This study provides a reference for anti-inflammatory treatment, especially for the treatment of gastrointestinal diseases.
Insights
This study combined antibacterial peptides with probiotic microcapsules to treat Vibrio parahaemolyticus infections. Microencapsulated probiotics demonstrated enhanced stability and therapeutic efficacy against gastrointestinal inflammation.
Area of Science:
- Microbiology
- Biotechnology
- Pharmacology
Background:
- Growing prevalence of drug-resistant bacteria necessitates novel therapeutic strategies.
- Investigating alternative treatments for gastrointestinal inflammation caused by Vibrio parahaemolyticus infection.
Purpose of the Study:
- To evaluate the efficacy of combining antibacterial peptides with microencapsulated probiotics for treating Vibrio parahaemolyticus-induced gastrointestinal inflammation.
- To assess the stability and therapeutic effects of Lacticaseibacillus casei CGMCC1.8727 microcapsules.
Main Methods:
- Probiotics (Lacticaseibacillus casei CGMCC1.8727) were encapsulated using anionic polysaccharides and chitosan.
- In vitro characterization included acid resistance and intestinal release tests.
- In vivo studies compared microencapsulated probiotics, free probiotics, and probiotics combined with the antibacterial peptide bomidin.
Main Results:
- Successful microencapsulation yielded particles of 2.04 ± 0.2743 mm.
- Microencapsulation significantly enhanced probiotic stability and activity under simulated gastrointestinal conditions compared to free probiotics.
- Microencapsulated probiotics exhibited superior in vivo therapeutic effects, with combined microcapsules and antimicrobial peptides accelerating bacterial elimination.
Conclusions:
- Microencapsulation improves probiotic stability and efficacy for gastrointestinal applications.
- The combination of microencapsulated probiotics and antibacterial peptides offers a promising therapeutic approach for bacterial infections.
- This strategy provides a valuable reference for developing anti-inflammatory treatments, particularly for gastrointestinal diseases.
