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Related Concept Videos

ATP Driven Pumps II: P-type Pumps01:34

ATP Driven Pumps II: P-type Pumps

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The P-type pumps are a large family of integral membrane transporter ATPases. They are divided into five major types based on substrate specificity, from I to V.
A typical P-type pump has three cytosolic domains: nucleotide-binding (N), phosphorylation (P), and activator (A) domains. These domains are connected to the membrane-spanning helices by short amino acid segments. ATP hydrolysis and covalent phosphoenzyme intermediate formation are crucial parts of the catalytic cycle. At the highly...
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ATP Driven Pumps III: V-type Pumps01:30

ATP Driven Pumps III: V-type Pumps

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V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
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Chemiosmosis01:32

Chemiosmosis

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Oxidative phosphorylation is a highly efficient process that generates large amounts of adenosine triphosphate (ATP), the basic unit of energy that drives many cellular processes. Oxidative phosphorylation involves two processes— the electron transport chain and chemiosmosis.
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons...
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Electron Transport Chains01:28

Electron Transport Chains

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The final stage of cellular respiration is oxidative phosphorylation that consists of two steps: the electron transport chain and chemiosmosis. The electron transport chain is a set of proteins found in the inner mitochondrial membrane in eukaryotic cells. Its primary function is to establish a proton gradient that can be used during chemiosmosis to produce ATP and generate electron carriers, such as NAD+ and FAD, that are used in glycolysis and the citric acid cycle.
The ETC is comprised of...
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Electron Transport Chain Components01:29

Electron Transport Chain Components

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The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...
126
ATP Driven Pumps I: An Overview01:27

ATP Driven Pumps I: An Overview

8.4K
ATP-driven pumps, also known as transport ATPases, are integral membrane proteins. They have binding sites for ATP located on the membrane's cytosolic side and the ion-conducting domain in the transmembrane region. These pumps use the free energy released from ATP hydrolysis to move the solutes across cell membranes against an electrochemical gradient.
There are four main types of ATP-driven pumps - P-type, V-type, F-type, and ABC transporter. All these pumps are of varying complexities and...
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Related Experiment Video

Updated: Aug 22, 2025

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Dense Electrode Layers-Supported Microtubular Oxygen Pump.

Alexey Nikonov1, Nikita Pavzderin1, Vladimir Khrustov1

  • 1Institute of Electrophysics of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg 620016, Russia.

Membranes
|November 11, 2022
PubMed
Summary

This study presents a novel microtubular solid oxide oxygen pump using cerium gadolinium oxide (GDC) electrolyte and lanthanum strontium cobalt ferrite (LSCF)-GDC electrodes. The developed oxygen pump demonstrates high efficiency and thermal shock resistance for potential medical applications.

Keywords:
co-sinteringdense composite electrode layermicrotubular celloxygen pumppolarization resistancethin GDC electrolyte

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Area of Science:

  • Electrochemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Oxygen pumps are electrochemical devices with medical applications.
  • Solid oxide oxygen pumps utilize specific ceramic electrolytes and electrode materials.
  • Optimizing electrode performance and structural integrity is crucial for device efficiency.

Purpose of the Study:

  • To develop and test a microtubular solid oxide oxygen pump with a cerium gadolinium oxide (GDC) electrolyte.
  • To utilize lanthanum strontium cobalt ferrite (LSCF)-GDC composite for dense supporting electrodes.
  • To evaluate the oxygen productivity, energy consumption, and thermal shock resistance of the developed pump.

Main Methods:

  • Fabrication of microtubular oxygen pumps using isostatic pressing and co-sintering of GDC tapes.
  • Preparation of dense LSCF-GDC composite electrodes to minimize polarization resistance.
  • Characterization using impedance spectroscopy and measurement of oxygen productivity at elevated temperatures.

Main Results:

  • Dense LSCF-GDC electrodes exhibited 2.5-5 times lower polarization resistance compared to standard porous electrodes.
  • Co-sintering at 1200 °C using nanosized GDC powder prevented chemical interactions between GDC and LSCF.
  • Achieved specific oxygen productivity of 0.29 L·h⁻¹·cm⁻² at 800 °C and 1.26 A·cm⁻².
  • Demonstrated low energy consumption (2.3 W·h per liter of oxygen) and high thermal shock resistance.

Conclusions:

  • The developed microtubular solid oxide oxygen pump with dense electrodes offers high efficiency and stability.
  • The fabrication method allows for co-sintering at temperatures that prevent detrimental chemical interactions.
  • The device shows significant potential for medical applications requiring efficient oxygen generation.