Related Experiment Video
Updated: Jun 22, 2026
Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
Published on: January 5, 2017
Efficacy of a Probiotic and Herbal Supplement in Models of Lung Inflammation
Nancy M Wenger1, Luhua Qiao2, Teodora Nicola2
1ResBiotic Nutrition Inc., Birmingham, AL 35203, USA.
Background:
Gut microbiome dysbiosis is associated with lung disease through the gut-lung axis. Abundant proteobacteria increase MMP-9 and contribute to tissue proteolysis followed by neutrophil recruitment, lung tissue injury, and perpetuation of chronic lung disease. We sought to determine if a scientifically formulated probiotic and herbal supplement could attenuate neutrophilic inflammation and improve lung structure and function in models of lung inflammation.
Methods:
For in vitro experiments, epithelial cells exposed to proteobacteria were treated with resB-a blend of three probiotic Lactobacillus strains and turmeric, holy basil, and vasaka herbal extracts. For in vivo experimentation, mice exposed to pulmonary proteobacteria-derived lipopolysaccharide were treated by gavage with resB.
Results:
In vitro, the bacterial and herbal components of resB decreased activity of the MMP-9 pathway. Mice exposed to LPS and pre- and post-treated with resB had decreased neutrophil recruitment and inflammatory biomarkers in bronchoalveolar lavage fluid, serum, and lung tissue compared to untreated mice.
Conclusions:
This study describes the mechanisms and efficacy of probiotic and herbal blend in pre-clinical models of lung injury and inflammation.
Insights
A probiotic and herbal supplement (resB) reduced lung inflammation and injury in preclinical models. This blend decreased neutrophil recruitment and inflammatory markers, suggesting potential for treating lung diseases linked to gut microbiome imbalances.
Area of Science:
- Microbiology
- Immunology
- Pharmacology
Background:
- Gut microbiome dysbiosis is linked to lung disease via the gut-lung axis.
- Elevated proteobacteria promote matrix metalloproteinase-9 (MMP-9) activity, leading to tissue damage and inflammation.
- This contributes to neutrophil recruitment, lung injury, and chronic lung disease progression.
Purpose of the Study:
- To investigate if a probiotic and herbal supplement (resB) can reduce neutrophilic inflammation.
- To determine if resB can improve lung structure and function in inflammation models.
Main Methods:
- In vitro: Epithelial cells exposed to proteobacteria were treated with resB (probiotic Lactobacillus strains, turmeric, holy basil, vasaka).
- In vivo: Mice exposed to lipopolysaccharide (LPS) were treated with resB via gavage.
Main Results:
- In vitro, resB components inhibited MMP-9 pathway activity.
- In vivo, resB treatment decreased neutrophil recruitment and inflammatory biomarkers in lung lavage fluid, serum, and tissue.
- These effects were observed in mice pre- and post-LPS exposure.
Conclusions:
- The study elucidates the mechanisms by which resB modulates lung inflammation.
- resB demonstrated efficacy in preclinical models of lung injury and inflammation.
- This probiotic and herbal blend shows potential for therapeutic intervention in lung diseases.
