Electrochemical Sensor for Bilirubin Detection Using Paper-Based Screen-Printed Electrodes Functionalized with Silver...

Area of Science:

• Electrochemistry
• Biosensors
• Nanomaterials

Background:

• Bilirubin is a crucial biomarker for diagnosing hemolytic diseases and jaundice.
• Accurate bilirubin measurement is vital for patient health, necessitating portable and affordable diagnostic tools.
• Current diagnostic methods may lack the portability and cost-effectiveness required for point-of-care applications.

Purpose of the Study:

• To develop a novel, inexpensive, and miniature electrochemical biosensor for selective bilirubin detection.
• To utilize paper-based screen-printed carbon electrodes modified with silver nanoparticles (AgNPs) and bilirubin oxidase.
• To establish a point-of-care diagnostic tool for jaundice and related conditions.

Main Methods:

• Fabrication of paper-based screen-printed carbon electrodes modified with AgNPs.
• Immobilization of bilirubin oxidase onto the modified electrodes to create an electrochemical biosensor.
• Characterization of AgNPs using UV-Vis Spectroscopy and Transmission Electron Microscopy (TEM).
• Electrochemical analysis using Cyclic Voltammetry (CV) and Linear Sweep Voltammetry (LSV).

Main Results:

• The developed paper-based sensor demonstrated optimal performance for bilirubin detection.
• Achieved a broad linear range of 1 to 9 µg/mL for bilirubin quantification.
• Established a low limit of detection (LOD) of 1 µg/mL.
• Confirmed sensor viability through testing with artificial blood serum samples.

Conclusions:

• A cost-effective, miniature electrochemical sensor for bilirubin detection has been successfully developed.
• The paper-based biosensor offers a promising platform for portable and accessible point-of-care diagnostics.
• This technology can aid in the early and efficient diagnosis of jaundice and hemolytic diseases.