WWOX Modulates ROS-Dependent Senescence in Bladder Cancer

Related Experiment Video

Updated: Aug 22, 2025

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.2K

Ching-Wen Liu1, Po-Hen Chen2, Tsan-Jung Yu3

  • 1Department of Senior Citizen Health Service and Management, Yuh-Ing Junior College of Health Care and Management, Kaohsiung 80776, Taiwan.

Molecules (Basel, Switzerland)
|November 11, 2022
PubMed

Insights

The WW domain-containing oxidoreductase (WWOX) gene promotes bladder cancer cell senescence by increasing reactive oxygen species (ROS). WWOX inhibits tumor growth, suggesting therapeutic potential for bladder cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cellular Senescence

Background:

  • WW domain-containing oxidoreductase (WWOX) is a tumor suppressor gene frequently lost in cancers.
  • Reactive oxygen species (ROS) are critical signaling molecules in inducing cell senescence.

Purpose of the Study:

  • To investigate the role of WWOX in regulating ROS and cell senescence.
  • To explore the mechanism of WWOX in bladder cancer progression.

Main Methods:

  • Utilized AY-27 rat bladder tumor cell line and F344 orthotopic bladder tumor models.
  • Employed WWOX-overexpressing lentivirus (LV-WWOX) to assess senescence induction.
  • Analyzed senescence markers, ROS generation, and cell cycle regulators (p21/p27).

Main Results:

  • LV-WWOX significantly induced cellular senescence, including SASP formation, enlarged morphology, and SA-β-Gal staining.
  • WWOX-induced senescence was dependent on increased ROS generation, activating p21/p27.
  • LV-WWOX inhibited tumor size by 30.49% in vivo by activating cellular senescence and enhancing p21 expression.

Conclusions:

  • WWOX acts as a pro-senescence gene in bladder cancer through ROS induction.
  • WWOX demonstrates therapeutic potential for bladder cancer by inhibiting tumor growth.
  • Further research into WWOX's therapeutic implications in bladder cancer is warranted.
Keywords:
WWOXbladder cancerreactive oxygen speciessenescence

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