Related Experiment Video
Updated: Aug 22, 2025
09:32
Fabrication of a Crystalline Nanocellulose Embedded Agarose Biomaterial Ink for Bone Marrow-Derived Mast Cell Culture
Published on: May 11, 2021
3.3K
3D-Printed Pectin/Carboxymethyl Cellulose/ZnO Bio-Inks: Comparative Analysis with the Solution Casting Method
Yeon Ho Kim1,2, Ruchir Priyadarshi1, Jin-Wook Kim2
1Department of Food and Nutrition, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.
Polymers
|November 11, 2022
Summary
3D bioprinting offers a superior method for creating pectin, carboxymethyl cellulose, and ZnO nanoparticle bio-ink films. These 3D-printed films exhibit enhanced properties and antimicrobial activity, proving safe for cell proliferation.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Developing advanced bio-inks is crucial for fabricating functional materials.
- Pectin (Pec), carboxymethyl cellulose (CMC), and zinc oxide (ZnO) nanoparticles are promising bio-ink components.
- 3D bioprinting offers precise fabrication control for complex structures.
Purpose of the Study:
- To compare the efficacy of 3D bioprinting versus solution casting for creating Pec/CMC/ZnO bio-ink films.
- To evaluate the physical, mechanical, barrier, and antimicrobial properties of the fabricated films.
- To assess the cytocompatibility and cellular effects of the bio-inks.
Main Methods:
- Fabrication of pectin/carboxymethyl cellulose/ZnO nanoparticle (Pec/CMC/ZnO) films using solution casting and 3D bioprinting.
- Surface morphology analysis using Field Emission Scanning Electron Microscopy (FE-SEM).
- Assessment of water vapor barrier, hydrophobicity, mechanical strength, and antimicrobial activity against *S. aureus* and *E. coli* O157:H7.
- Cytotoxicity and fibroblast proliferation assays using normal human dermal fibroblast (NDFB) cells.
Main Results:
- 3D bioprinted Pec/CMC/ZnO films exhibited denser and more compact surfaces than solution-cast films.
- Enhanced water vapor barrier, hydrophobicity, and mechanical properties were observed in 3D bioprinted films.
- Significant antimicrobial activity against *S. aureus* and *E. coli* O157:H7 within 12 hours was demonstrated by 3D bioprinted films.
- The bio-inks showed no cytotoxicity and promoted fibroblast proliferation in NDFB cells.
Conclusions:
- 3D bioprinting is a superior and practical alternative to solution casting for fabricating advanced biopolymer films.
- Pec/CMC/ZnO bio-inks are suitable for 3D printing functional films and scaffolds for biomedical applications.
- The developed 3D bioprinted films possess desirable properties for potential use in tissue engineering and regenerative medicine.
