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Updated: Aug 22, 2025

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
Published on: April 9, 2014
Reduction in required volume of imaging data and image reconstruction time for adaptive-illumination Fourier
Jiaxiong Luo1, Haishu Tan1, Ruofei Wu1
1School of Physics and Optoelectronic Engineering, Foshan University, Foshan, China.
Abstract:
Fourier ptychographic microscopy (FPM) is a promising super-resolution computational imaging technology. It stitches a series of low-resolution (LR) images in the Fourier domain by an iterative method. Thus, it obtains a large field of view and high-resolution quantitative phase images. Owing to its capability to perform high-spatial bandwidth product imaging, FPM is widely used in the reconstruction of conventional static samples. However, the influence of the FPM imaging mechanism limits its application in high-speed dynamic imaging. To solve this problem, an adaptive-illumination FPM scheme using regional energy estimation is proposed. Starting with several captured real LR images, the energy distribution of all LR images is estimated, and select the measurement images with large information to perform FPM reconstruction. Simulation and experimental results show that the method produces efficient imaging performance and reduces the required volume of data to more than 65% while ensuring the quality of FPM reconstruction.

