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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: Aug 22, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives.

Tao Huang1, Rui Zhang1, Jinming Li1

  • 1National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, PR China; Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, PR China; Beijing Engineering Research Center of Laboratory Medicine, Beijing Hospital, Beijing, PR China.

Journal of Advanced Research
|November 11, 2022
PubMed
Summary

CRISPR-Cas technology offers a promising alternative for rapid, sensitive, and cost-effective pathogen detection. This review explores its advancements, overcoming limitations of current methods like polymerase chain reaction for improved diagnostics.

Keywords:
CRISPR-CasPathogen detectionPoint-of-care testingStandardized testing

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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Infectious Disease Diagnostics

Background:

  • Early detection of pathogen-associated diseases is crucial for effective treatment.
  • Current diagnostic methods, such as polymerase chain reaction (PCR), face challenges including high cost, long cycles, and extensive technical requirements.
  • There is a critical need for rapid, specific, sensitive, and cost-effective pathogen detection technologies.

Purpose of the Study:

  • To review and highlight technical advancements in CRISPR-Cas systems for pathogen detection.
  • To provide an outlook on future developments, multi-application scenarios, and clinical translation of CRISPR-Cas-based diagnostics.
  • To discuss the potential of CRISPR-Cas technology to overcome limitations of existing diagnostic tools.

Main Methods:

  • Review of scientific literature on CRISPR-Cas systems applied to nucleic acid detection.
  • Analysis of CRISPR-Cas9 specificity and the collateral cleavage activity of CRISPR-Cas12, Cas13, and Cas14.
  • In-depth discussion of various CRISPR-Cas-based pathogen detection methods.

Main Results:

  • CRISPR-Cas systems demonstrate high specificity, versatility, and rapid detection cycles for nucleic acids.
  • CRISPR-Cas9's precise targeting and the collateral cleavage activity of other Cas enzymes show significant promise for diagnostic applications.
  • CRISPR-Cas-based methods offer potential for sensitive, specific, cheap, and portable clinical detection of pathogens.

Conclusions:

  • CRISPR-Cas technology presents a powerful platform for developing ideal diagnostic tools for pathogen detection.
  • Despite existing challenges, the capabilities of CRISPR-Cas-mediated solutions are poised to revolutionize infectious disease diagnostics.
  • Further development and clinical translation of CRISPR-Cas systems are expected to significantly improve pathogen detection.