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Microbiological method for assaying lincomycin in animal feed: collaborative study.

A W Neff, R W Thomas

    Journal - Association of Official Analytical Chemists
    |September 1, 1978
    PubMed
    Summary
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    A collaborative study validated a microbiological assay for lincomycin detection in swine feed and supplements. The assay demonstrated reliable lincomycin quantification across various feed types and fortification levels.

    Area of Science:

    • Analytical Chemistry
    • Microbiology
    • Animal Science

    Background:

    • Accurate quantification of antimicrobial agents like lincomycin in animal feed is crucial for regulatory compliance and animal health.
    • Existing methods may lack the precision or scope for diverse feed matrices and fortification levels.

    Purpose of the Study:

    • To collaboratively study and validate a microbiological assay for determining lincomycin concentrations in swine feed, supplements, and vitamin-mineral premixes.
    • To assess the assay's reliability across a wide range of lincomycin fortification levels.

    Main Methods:

    • A collaborative study involving 16 laboratories.
    • Two distinct sample preparation methods were employed based on lincomycin concentration.
    • Microbiological assay technique utilized for lincomycin quantification.

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    Main Results:

    • High mean recoveries were achieved: 112.2% (102.3–123.5%) at lower levels and 104.4% (100.0–107.7%) at higher levels.
    • Statistical analysis showed no significant differences in mean recoveries between collaborators for both methods.
    • The assay proved effective for lincomycin determination in complete feed, feed supplements, and vitamin-mineral premixes.

    Conclusions:

    • The validated microbiological assay is suitable for the accurate determination of lincomycin in various swine feed matrices.
    • The method's reliability across different fortification levels supports its adoption for routine analysis.
    • The assay has been officially adopted as a first action method.