Hydrogen sulfide and nitric oxide regulate the adaptation to iron deficiency through affecting Fe homeostasis and...

Iron (Fe) is a vital microelement required for the growth and development of plants. Hydrogen sulfide (H₂S) and nitric oxide (NO), as messenger molecules, participated in the regulation of plant physiological processes. Here, we studied the interaction effects of H₂S and NO on the adaptation to Fe deficiency in Glycine max L. Physiological, biochemical and molecular approaches were conducted to analyze the role of H₂S and NO in regulating the adaptation to Fe deficiency in soybean. We found that H₂S and NO had obvious rescuing function on the Fe deficiency-induced the plant growth inhibition, which was significantly correlated with the increase in Fe content in the leaves, stems, and roots of soybean. Meanwhile, H⁺-flux, ferric chelate reductase (FCR) activity, and root apoplast Fe content were significantly affected by H₂S and NO. Under Fe deficiency conditions NO and H₂S regulated the expression of genes related to Fe homeostasis. Moreover, photosynthesis (Pn) and photosystem II (PSII) efficiency were enhanced by H₂S and NO, and thiol redox modification was important for regulating the adaptation of Fe deficiency. The aforementioned affirmative influences caused by H₂S and NO were also totally reversed by cPTIO (a NO scavenger). Our results suggested that H₂S might act upstream of NO in response to Fe deficiency by affecting the Fe homeostasis enzyme activities and gene expression, and by promoting Fe accumulation in plant tissues as well as by enhancing thiol redox modification and photosynthesis in soybean plants.