Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomics02:02

Genomics

36.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.9K
Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.4K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.4K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.5K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
23.5K
Epistasis Analysis01:09

Epistasis Analysis

5.1K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
5.1K
Histone Modification02:32

Histone Modification

13.6K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitotic Cdc42 waves encode PI(3,4)P<sub>2</sub> signaling and Golgi morphological state to control spindle scaling.

Science advances·2026
Same author

Implementation of an adaptive-optics assisted isoSTED nanoscope.

Nature protocols·2026
Same author

Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells.

bioRxiv : the preprint server for biology·2026
Same author

pan-ASLM: Axially Swept Light Sheet Microscopy for Fast and High-Resolution Imaging of Expanded Samples.

Npj imaging·2026
Same author

Oligomeric assembly of the gatekeeper InvE orchestrates hierarchical type III protein secretion in <i>Salmonella</i> Typhimurium.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

All-optical visualization of specific molecules in the ultrastructural context of brain tissue.

Nature biotechnology·2025
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026
See all related articles

Related Experiment Video

Updated: Aug 22, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K

Omics goes spatial epigenomics.

Florian Schueder1, Joerg Bewersdorf2

  • 1Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA; Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, CT, USA.

Cell
|November 11, 2022
PubMed
Summary
This summary is machine-generated.

Spatial omics technologies provide detailed molecular data within tissues. New epigenetic multiplexing methods like Multiplexed Error-robust FISH (MERFISH) enable studying gene expression regulation in specific tissue regions.

More Related Videos

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.3K
An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

10.5K

Related Experiment Videos

Last Updated: Aug 22, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

2.3K
An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
10:41

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

10.5K

Area of Science:

  • Molecular biology
  • Genomics
  • Cell biology

Background:

  • Spatial omics techniques offer high-resolution molecular data from tissues.
  • Understanding cell identity and function relies on molecular profiling within spatial contexts.
  • Epigenomic regulation plays a crucial role in gene expression but requires spatially resolved analysis.

Purpose of the Study:

  • To introduce and demonstrate an epigenetic multiplexing approach for spatial gene expression analysis.
  • To enable the study of epigenomic regulation in specific tissue regions.
  • To advance the capabilities of spatial omics for understanding cellular processes.

Main Methods:

  • Multiplexed Error-robust FISH (MERFISH) technique.
  • High-throughput molecular profiling.
  • Spatially resolved data acquisition in tissues.

Main Results:

  • MERFISH allows for multiplexed, error-robust detection of RNA molecules.
  • The method provides spatially resolved information on gene expression.
  • Enables the investigation of epigenomic regulation in a tissue-specific manner.

Conclusions:

  • Epigenetic multiplexing approaches like MERFISH are powerful tools for spatial transcriptomics.
  • This technique facilitates the study of gene expression regulation within defined tissue microenvironments.
  • Advances in spatial omics are crucial for dissecting complex biological systems.