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Updated: Aug 22, 2025
Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
Published on: August 19, 2015
In vitro biodegradation, cytotoxicity, and biocompatibility of polylactic acid/napier cellulose nanofiber scaffold
R Revati1, M S Abdul Majid2, M J M Ridzuan2
1Faculty of Mechanical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Pauh Putra Campus, Arau 02600, Perlis, Malaysia; Faculty of Electronic Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Pauh Putra Campus, Arau 02600, Perlis, Malaysia.
Abstract:
This study aimed to evaluate the bioactivities and biocompatibilities of porous polylactic acid (PLA) reinforced with cellulose nanofiber (CNF) scaffolds. The in vitro degradation behaviors of the porous PLA/CNF scaffolds were systematically measured for up to 8 weeks in a phosphate-buffered saline medium at 37 °C. The reinforcement of CNF resisted the biodegradation of the scaffolds. The in vitro cytotoxicity and biocompatibility of the scaffolds were determined using the Beas2B American Type Culture Collection cells. The 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide cytotoxicity and proliferation tests showed that the scaffolds were non-toxic, and epithelial cells grew well on the scaffold after 7 days of culture, whereas the percentage of cell proliferation on the PLA/CNF15 scaffold was the largest, 130 %. A scratch wound-healing assay was performed to evaluate the suitability of the scaffolds for cell migration. The results demonstrated that the scaffolds exhibited good cell migration towards nearly complete wound closure.
