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Related Experiment Videos

dnaT, dominant conditional-lethal mutation affecting DNA replication in Escherichia coli.

C A Lark, J Riazi, K G Lark

    Journal of Bacteriology
    |December 1, 1978
    PubMed
    Summary

    Bacteria normally stop DNA replication without protein synthesis. Mutants were found that always terminate replication under these conditions, impacting cell division and growth.

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    Isolation of sodium dependent variants from haploid soybean cell culture.

    Plant cell reports·2013

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Bacteria typically halt DNA replication when protein synthesis is inhibited.
    • Certain conditions, like thymine starvation or nutrient-rich medium shifts, can override this, allowing replication to continue without protein synthesis.

    Purpose of the Study:

    • To identify and characterize bacterial mutants that exhibit obligatory termination of DNA replication.
    • To investigate the genetic basis and phenotypic consequences of mutations affecting DNA replication termination.

    Main Methods:

    • Isolation and characterization of conditional lethal mutants (dnaT1, dnaT2).
    • Transductional mapping to identify linked genetic markers (serB, dnaC).
    • Analysis of mutant phenotypes including DNA replication, cell division, and dominance.
    • Assessment of episomal stability in mutants.

    Main Results:

    • Identified dnaT1 and dnaT2 mutants that obligatorily terminate DNA replication when protein synthesis is absent.
    • These mutations are contransduced with serB and dnaC and also affect cell division.
    • The mutant phenotype, including obligatory termination and temperature sensitivity, is transdominant to the wild-type.
    • Mutant episomes demonstrated instability.

    Conclusions:

    • The dnaT mutation confers a dominant phenotype affecting DNA replication termination and cell division.
    • The findings support theoretical models predicting the existence of dominant mutations in chromosome termination pathways.
    • These mutants provide valuable tools for studying the mechanisms of bacterial DNA replication termination.

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