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Updated: Aug 22, 2025
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
Published on: August 30, 2017
A NIR light gated targeting nanoprobe based on DNA-modified upconversion nanoparticles with antifouling properties
Yan Gong1, Wei Yuan1, Fuxiang Song1
1Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Abstract:
The overexpression of microRNA-21 is closely related to many human diseases, so the development of probes for this bio-marker will contribute to the diagnosis. In this scheme, a novel ratiometric upconversion nanoprobe based on fluorescence resonance energy transfer (FRET) was developed for the analysis of microRNA-21. This nanosystem is composed of upconversion nanoparticles (UCNPs), PEG with antifouling performance and targeted folic acid (FA). Er3+ doped UCNPs were used as energy donors and carboxy-X-rhodamine (ROX) acted as energy receptors. MicroRNA-21 was visually analyzed by ratiometric upconversion luminescence (548 nm/616 nm), and the limit of detection (LOD) was quantified to be 9.95 pM. This nanocomposite can not only realize the detection and imaging for microRNA-21 in cells, but also visualize microRNA-21 level in vivo by upconversion luminescence due to the deep tissue penetration of UCNPs.
