Understand the antibacterial behavior and mechanism of hydrothermal wastewater

Unlocking the antibacterial potential is an emerging strategy to valorizing the toxic wastewater from hydrothermal liquefaction (HTL). Here, we investigated the response and biological mechanism of antibacterial properties of HTL wastewater. Four different biowastes i.e. microalgae, cornstalk, cow manure and swine manure were used as the feedstock of HTL to create wastewater with diverse molecule spectrum, whereas ten strains i.e. five gram-positive strains and five gram-negative strains were employed to represent typical pathogenic microorganism. HTL wastewater exhibited antibacterial potential and obvious reduction on cell viability at high inclusion ratio, although the minimum inhibitory concentration (MIC) and cell response intensity varied depending on different HTL feedstocks and strain species. The decreased ATP generation and increased H₂O₂ accumulation in treated cells further confirmed the inhibition of HTL wastewater on the cell metabolism. The antibacterial mechanism of HTL wastewater was confirmed, including damage to biomolecules or membranes, depletion of crucial components, disruption of metabolic circuits and imbalance of creation of redox cofactor. The complex compounds in HTL wastewater were probably attributed to the multiple inhibition pathways and the relationship among those multiple pathways was speculated. The present study contributes to the mechanism analysis of complex compound mixture and bactericide characteristics of HTL wastewater.