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Morphological structure of rat tongue using light and scanning electron microscopy
Beste Demirci1, Banu Kandil2, Süleyman Yüksel1
1Department of Anatomy, Veterinary Faculty, Kastamonu University, Kastamonu, Türkiye.
Microscopy Research and Technique
|November 12, 2022
Summary
This study details the tongue morphology of Wistar rats, commonly used in research. Formalin fixation is confirmed as a viable method for scanning electron microscope (SEM) analysis of rat tongue structure.
Area of Science:
- Veterinary Anatomy
- Comparative Morphology
- Scanning Electron Microscopy
Background:
- Rats are crucial models in biomedical research.
- Tongue epithelium structure varies with anatomy and diet.
- Morphological diversity aids animal adaptation.
Purpose of the Study:
- To elucidate the tongue morphology of Wistar rats.
- To validate formalin fixation for SEM analysis.
- To compare Wistar rat tongue anatomy with other species.
Main Methods:
- Investigated seven male Wistar rat tongues.
- Utilized scanning electron microscopy (SEM) with formalin and glutaraldehyde fixation.
- Performed histological evaluation using triple staining.
Main Results:
- Identified three main tongue components: apex, body, and root.
- Described the rounded, bifurcated apex.
- Documented filiform and three types of gustatory papillae (fungiform, vallate, foliate) on the dorsal surface.
Conclusions:
- Wistar rat tongue morphology is well-defined.
- Formalin fixation is suitable for SEM morphological studies.
- This research provides a basis for comparative tongue anatomy studies.
