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Updated: Aug 22, 2025

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins
Harumi Kawaki1,2, Satoshi Kubota3, Masaharu Takigawa4
1Department of Chemistry, Asahi University School of Dentistry, Gifu, Japan. kawaki@dent.asahi-u.ac.jp.
Abstract:
The method of labeling proteins of interest with fluorescent dyes that can specifically stain organelles in living cells provides a tool for investigating various cellular processes under a microscope. Visualization (imaging) of the cells using fluorescence has many advantages, including the ability to stain multiple cell organelles and intracellular proteins simultaneously and discriminately, and is used in many research fields. In this chapter, we describe the observation of cell organelles using fluorescence staining to analyze the functions of CCN family proteins involved in various cellular events.

