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Developmental validation of STRmix™ NGS, a probabilistic genotyping tool for the interpretation of autosomal STRs
Kevin Cheng1, Jo-Anne Bright1, Hannah Kelly1
1Institute of Environmental Science and Research Limited, Private Bag 92021, Auckland 1142, New Zealand.
Abstract:
We describe the developmental validation of the probabilistic genotyping software - STRmix™ NGS - developed for the interpretation of forensic DNA profiles containing autosomal STRs generated using next generation sequencing (NGS) also known as massively parallel sequencing (MPS) technologies. Developmental validation was carried out in accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM) Guidelines for the Validation of Probabilistic Genotyping Systems and the International Society for Forensic Genetics (ISFG) recommendations and included sensitivity and specificity testing, accuracy, precision, and the interpretation of case-types samples. The results of developmental validation demonstrate the appropriateness of the software for the interpretation of profiles developed using NGS technology.
