Related Experiment Video

Updated: Aug 22, 2025

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

38.9K

Andrographolide and pterostilbene inhibit adipocyte differentiation by downregulating PPARγ through different

Saurabh D Kalamkar1, Ganesh Suraj Bose2, Saroj Ghaskadbi1

  • 1Department of Zoology, Savitribai Phule Pune University, Pune, India.

Natural Product Research
|November 14, 2022
PubMed

Insights

Two natural compounds, andrographolide (AN) and pterostilbene (PT), inhibit fat cell formation (adipogenesis) by blocking key molecular pathways and reducing reactive oxygen species (ROS). This research clarifies their anti-adipogenic mechanisms.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Adipogenesis is the process of stem cell differentiation into adipocytes, regulated by hormones and nutrients.
  • Natural compounds are explored for anti-adipogenic effects via apoptosis, blocked differentiation, or inhibited triglyceride synthesis.
  • Understanding the molecular mechanisms of these compounds is crucial for developing anti-obesity strategies.

Purpose of the Study:

  • To elucidate the molecular mechanisms of andrographolide (AN) and pterostilbene (PT) in inhibiting human mesenchymal stem cell (MSC) differentiation into adipocytes.
  • To investigate the role of AN and PT in regulating key adipogenic transcription factors and signaling pathways.

Main Methods:

  • Human MSCs were induced to differentiate into adipocytes.
  • The expression levels of microRNAs (e.g., miR27a) and proteins (e.g., SIRT1, PPARγ, SREBP1c) were analyzed.
  • Reactive oxygen species (ROS) production during differentiation was measured.

Main Results:

  • Andrographolide (AN) upregulated miR27a, while pterostilbene (PT) upregulated SIRT1.
  • Both AN and PT inhibited the expression of key adipogenic transcription factors, including PPARγ (peroxisome proliferator-activated receptor gamma) and SREBP1c (sterol regulatory element-binding protein 1c).
  • AN and PT effectively blocked the surge of reactive oxygen species (ROS) during adipocyte differentiation.

Conclusions:

  • Andrographolide and pterostilbene demonstrate significant anti-adipogenic activity through distinct molecular pathways.
  • These phytocompounds inhibit adipogenesis by modulating miR27a, SIRT1, and crucial transcription factors (PPARγ, SREBP1c), and by reducing ROS.
  • The findings provide a molecular basis for the potential therapeutic use of AN and PT in managing obesity and related metabolic disorders.
Keywords:
AdipogenesisPPARγROSandrographolidepterostilbene

More Related Videos

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

1.5K
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.2K

Related Concept Videos

GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.8K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.6K
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.5K