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Updated: Aug 22, 2025

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
Published on: May 12, 2017
An RNA granule for translation quality control in Saccharomyces cerevisiae
James S Dhaliwal1, Cristina Panozzo2, Lionel Benard2
1Department of Biology, Concordia University, 7141 Sherbrooke W, Montreal, Quebec, Canada, H4B 1R6.
Abstract:
Cytoplasmic RNA granules compartmentalize phases of the translation cycle in eukaryotes. We previously reported the localization of oxidized RNA to cytoplasmic foci called oxidized RNA bodies (ORBs) in human cells. We show here that ORBs are RNA granules in Saccharomyces cerevisiae. Several lines of evidence support a role for ORBs in the compartmentalization of no-go decay and ribosome quality control, the translation quality control pathways that recognize and clear aberrant mRNAs, including those with oxidized bases. Translation is required by these pathways and ORBs. Translation quality control factors localize to ORBs. A substrate of translation quality control, a stalled mRNA-ribosome-nascent-chain complex, localizes to ORBs. Translation quality control mutants have altered ORB numbers, sizes or both. In addition, we identify 68 ORB proteins by immunofluorescence staining directed by proteomics, which further support their role in translation quality control and reveal candidate new factors for these pathways.
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