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Updated: Aug 22, 2025
Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Generation of Murine T Cell Effector Populations In Vitro
1Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. melanie.vacchio@nih.gov.
Abstract:
Expansion of T cell subsets in vitro is a valuable tool for exploration of effector function and differentiation. Here we provide protocols for in vitro differentiation of CD4 and CD8 T cell subsets from naïve T cells for functional studies.
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