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Related Experiment Video

Updated: Aug 22, 2025

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

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Published on: September 13, 2018

Cytoskeletal protein SPTA1 mediating the decrease in erectile function induced by high-fat diet via Hippo signaling

Ying Chen^1,2, Lei Wang³, Zhan-Sen Huang¹

¹Department of Infertility and Sexual Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

|November 14, 2022

Summary

This summary is machine-generated.

High-fat diets decrease erectile function by down-regulating spectrin alpha, erythrocytic 1 (SPTA1), which may trigger cell pyroptosis and activate the Hippo pathway. These findings offer new therapeutic targets for improving erectile function.

Keywords:

Hippo signalling pathway SPTA1 cell pyroptosis erectile function decreased high-fat diet

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Area of Science:

Urology
Molecular Biology
Cell Biology

Background:

High-fat diet (HFD)-induced erectile dysfunction mechanisms remain unclear.
Spectrin alpha, erythrocytic 1 (SPTA1), a cytoskeletal protein, influences cell growth via the Hippo signaling pathway, but its role in erectile function is unelucidated.

Purpose of the Study:

To investigate the role of SPTA1 in HFD-induced erectile dysfunction.

Main Methods:

Transcriptomic analysis of penile tissues from mice on normal and HFD.
Verification of SPTA1 expression in rat penile tissues and in vitro cell models (high-glucose treated CCECs and CCSMCs).
Single-cell sequencing to determine SPTA1 distribution and gene interference to explore mechanisms.

Main Results:

SPTA1 was identified as a down-regulated gene in HFD-induced erectile dysfunction.
SPTA1 protein expression decreased in HFD rat penile tissues and HG-treated CCECs/CCSMCs.
Interfering with SPTA1 in CCSMCs led to eNOS down-regulation and pyroptosis/Hippo pathway activation (YAP, Caspase-1, GSDMD, IL-18, IL-1β up-regulation).

Conclusions:

Down-regulation of SPTA1 in HFD may induce pyroptosis and erectile dysfunction via Hippo pathway activation.
SPTA1 modulation presents a potential therapeutic strategy for erectile dysfunction.