Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ki67 and Lymphovascular Invasion as Histopathological Predictors of Residual Cancer Burden After Neoadjuvant Chemotherapy in Breast Cancer: A Retrospective Study.

Diagnostics (Basel, Switzerland)·2026
Same author

Bridging functional bionanomaterials and the clinic: strategic communication as a translational enabler.

Journal of nanobiotechnology·2026
Same author

FACE-ing the future of single-pixel complex-field microscopy beyond the visible spectrum.

Light, science & applications·2025
Same author

Subcellular glycan-mannose receptor binding kinetics correlate with myeloid cell function.

Nature communications·2025
Same author

Methylarginine Levels in Chronic Inflammatory Skin Diseases-The Role of L-Arginine/Nitric Oxide Pathway.

Journal of clinical medicine·2025
Same author

Key Biomarker Correlations in Cutaneous Melanoma: Implications for Diagnostic, Prognostic, and Therapeutic Strategies-A Retrospective Single-Centered Study.

Medicina (Kaunas, Lithuania)·2025

Related Experiment Video

Updated: Aug 22, 2025

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.2K

Super-resolution re-scan second harmonic generation microscopy.

Stefan G Stanciu1, Radu Hristu1, George A Stanciu1

  • 1Center for Microscopy-Microanalysis and Information Processing, University Politehnica of Bucharest, 060042 Bucharest, Romania.

Proceedings of the National Academy of Sciences of the United States of America
|November 14, 2022
PubMed
Summary
This summary is machine-generated.

Super-resolved re-scan second harmonic generation (SHG) microscopy achieves resolution beyond the diffraction limit for collagen imaging. This work also introduces super-resolved re-scan two-photon excited fluorescence microscopy for advanced visualization.

Keywords:
second harmonic generationsuper-resolved optical imagingtwo-photon excited fluorescence

More Related Videos

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.5K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

2.6K

Related Experiment Videos

Last Updated: Aug 22, 2025

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.2K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.5K
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
09:14

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

2.6K

Area of Science:

  • Biomedical optics
  • Microscopy
  • Materials science

Background:

  • Second harmonic generation (SHG) microscopy enables label-free 3D tissue and material visualization, particularly for collagen.
  • Super-resolution advancements in fluorescence microscopy have not been matched in SHG imaging.

Purpose of the Study:

  • To demonstrate super-resolved re-scan SHG microscopy for enhanced collagen imaging.
  • To introduce super-resolved re-scan two-photon excited fluorescence microscopy.

Main Methods:

  • Implementation of super-resolved re-scan SHG microscopy.
  • Quantitative and qualitative analysis of resolution improvements on collagenous tissues.
  • Development of super-resolved re-scan two-photon excited fluorescence microscopy.

Main Results:

  • Super-resolved re-scan SHG microscopy provides a resolution advantage beyond the diffraction limit.
  • Demonstrated effectiveness on collagenous tissues.
  • Successfully introduced a novel super-resolution imaging modality.

Conclusions:

  • Super-resolved re-scan SHG microscopy significantly enhances imaging resolution for biological and material samples.
  • The developed techniques offer new possibilities for high-resolution imaging in various scientific fields.