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Control of Cell Geometry through Infrared Laser Assisted Micropatterning
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Controlling shape morphing and cell release in engineered living materials.

Laura K Rivera-Tarazona1, Manivannan Sivaperuman Kalairaj1, Tyler Corazao2

  • 1Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.

Biomaterials Advances
|November 14, 2022
PubMed
Summary
This summary is machine-generated.

Engineered living materials (ELMs) with acrylic hydrogels and E. coli show controlled bacterial release and volume changes. Material properties like elastic modulus influence these phenomena, enabling potential probiotic delivery for medical devices.

Keywords:
BacteriaCell deliveryEngineered living materialsHydrogelsShape change

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Area of Science:

  • Biomaterials Science
  • Synthetic Biology
  • Microbiology

Background:

  • Engineered living materials (ELMs) integrate living cells within synthetic matrices for novel functionalities.
  • Current research aims to develop ELM-based medical devices with tunable mechanical and bioactive properties.
  • Understanding cell-matrix interactions is crucial for controlling ELM behavior.

Purpose of the Study:

  • To fabricate and characterize multifunctional, stimuli-responsive ELMs using acrylic hydrogels and Escherichia coli.
  • To investigate the role of hydrogel mechanical properties and cell loading on ELM form changes and bacterial release.
  • To explore the potential of these prokaryotic ELMs for in vitro probiotic delivery against uropathogens.

Main Methods:

  • Fabrication of acrylic hydrogel-based ELMs with encapsulated E. coli.
  • Systematic variation of hydrogel elastic modulus and initial cell loading.
  • Quantification of bacterial release (colony forming units/mL) and material volume changes.
  • In vitro assessment of probiotic delivery efficacy against a uropathogen.

Main Results:

  • ELM volume changes exceeding 100% and significant bacterial release (~10^6 CFU/mL) were observed.
  • Increased hydrogel elastic modulus reduced both bacterial delivery and volume change.
  • Higher initial cell loading led to significantly increased bacterial delivery.
  • ELMs demonstrated potential for delivering a probiotic to inhibit uropathogen growth in vitro.

Conclusions:

  • Mechanical forces from cell proliferation within hydrogels govern ELM form changes and bacterial release.
  • Tunable control over bacterial delivery and material response is achievable by modulating hydrogel properties and cell density.
  • These findings support the development of ELMs for advanced biomedical applications, including targeted microbial modulation.