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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Spectrophotometry: Introduction01:16

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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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Related Experiment Video

Updated: Aug 22, 2025

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
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An open-source handheld spectrometer for colorimetric and fluorescence analyses.

Zhicheng Yu1, Ruidong Meng1, Suqi Deng1

  • 1Ministry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, South China Normal University, Guangzhou 510631, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|November 14, 2022
PubMed
Summary

A new handheld spectrometer, costing only $50, offers portable and wireless fluid sample analysis. This cost-effective device provides comparable sensitivity to lab instruments for various applications.

Keywords:
3D printing technologyOptical detectionPortable deviceWireless

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biotechnology

Background:

  • Traditional spectrometers are costly, bulky, and require stable power, limiting field applications.
  • On-site analysis is crucial for biological, environmental, and diagnostic fields.
  • Need for portable, affordable, and accessible analytical instrumentation.

Purpose of the Study:

  • To design and fabricate a wireless, cost-effective, open-source, handheld spectrometer.
  • To enable on-site colorimetric and fluorescence analyses of fluid samples.
  • To demonstrate the feasibility of using 3D printing for low-cost analytical devices.

Main Methods:

  • Utilized off-the-shelf electronics and 3D printing technology for fabrication.
  • Developed a compact device (5x5x8 cm, 130g) with a 405-690 nm detection range.
  • Integrated Bluetooth for wireless data transmission to an Android application (SpecAnalysis).

Main Results:

  • The fabricated handheld spectrometer costs approximately $50.
  • Successfully demonstrated optical detection of Cu(II), bovine serum albumin, and calf thymus DNA.
  • Achieved sensitivity and detection limits comparable to commercial research-grade instruments.

Conclusions:

  • The developed handheld spectrometer is a viable, low-cost alternative for on-site analysis.
  • The device's portability and wireless capabilities enhance accessibility for field applications.
  • Potential for broad application in detecting various substances beyond specific compound quantification.