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Stable nanovesicles formed by intrinsically planar bilayers.

Mariana Köber1, Sílvia Illa-Tuset2, Lidia Ferrer-Tasies3

  • 1Networking Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain; Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Cerdanyola del Vallès, Spain.

Journal of Colloid and Interface Science
|November 14, 2022
PubMed
Summary
This summary is machine-generated.

Cholesterol (CHOL) and cetyltrimethylammonium bromide (CTAB) form stable nanovesicles through molecular distortions, not membrane curvature. This discovery advances understanding of self-assembling nanovesicle formation and stability.

Keywords:
Composition asymmetryMolecular self-assemblyNanovesiclesQuatsomesVesicle stability

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Area of Science:

  • Supramolecular Chemistry
  • Materials Science
  • Biophysics

Background:

  • Quatsome nanovesicles self-assemble from cholesterol (CHOL) and cetyltrimethylammonium bromide (CTAB).
  • These nanovesicles exhibit remarkable long-term stability in size and morphology.
  • High intermolecular binding energies between CHOL and CTAB are observed.

Purpose of the Study:

  • To investigate the formation mechanism of CHOL/CTAB quatsomes.
  • To determine if CHOL/CTAB quatsomes meet the criteria for thermodynamically stable vesicles.
  • To elucidate the molecular organization within the quatsome membrane.

Main Methods:

  • Coarse-grain molecular dynamics simulations were employed.
  • Experimental validation included cryo-electron microscopy, X-ray scattering (SAXS/WAXS), and static light scattering (MALS).
  • Systematic studies assessed the thermodynamic stability of CHOL/CTAB quatsomes.

Main Results:

  • CHOL/CTAB quatsomes satisfy the requirements for thermodynamic stability.
  • Unlike catanionic vesicles, stability is not driven by bilayer leaflet asymmetry.
  • Formation involves the association of planar bilayers with defects, highlighting molecular distortions' role.

Conclusions:

  • CHOL/CTAB quatsomes are thermodynamically stable nanovesicles.
  • Molecular organization and orientation, leading to distortions, are key to their formation.
  • This mechanism offers a new perspective on stable nanovesicle self-assembly.