Related Experiment Video

Updated: Sep 27, 2026

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
10:18

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants

Published on: November 1, 2016

The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA

A Schön, G Krupp, S Gough

    Nature
    |July 17, 1986
    PubMed

    More Related Videos

    Studying Protein Import into Chloroplasts Using Protoplasts06:29

    Studying Protein Import into Chloroplasts Using Protoplasts

    Published on: December 10, 2018

    Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation07:58

    Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

    Published on: January 12, 2024

    Related Concept Videos

    Photosystem II01:22

    Photosystem II

    The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
    The pigment molecules are arranged across  two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment molecules...
    The Calvin Cycle01:40

    The Calvin Cycle

    OverviewOxygenic photosynthesis plays a central role in the global carbon and oxygen cycles. The carbohydrates produced support nearly all food webs, while the oxygen by‑product enables aerobic life.Light‑dependent and light‑independent reactionsPhotosynthesis occurs in two main stages, each in a different part of the chloroplast: light‑dependent reactions and light‑independent reactions, also called the Calvin‑Benson cycle or simply the Calvin cycle.Light‑dependent reactions take place in the...
    Protein Transport to the Stroma01:24

    Protein Transport to the Stroma

    Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
    Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
    Protein Transport to the Outer Chloroplast Membrane01:11

    Protein Transport to the Outer Chloroplast Membrane

    Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
    Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
    Protein Transport to the Thylakoids01:22

    Protein Transport to the Thylakoids

    Thylakoids are membrane-bound sac-like structures within the chloroplast that serve as sites for photosynthesis. Thylakoid lumen contains many electron transport proteins and is enclosed by a thylakoid membrane rich in the light-harvesting complex. Proteins targeted to the thylakoids are transported as precursors and are sorted by the general TOC/TIC import pathway. Once the precursor reaches the stroma, stromal processing peptidases remove their transit signal and expose thylakoid signal...
    Protein Transport to the Inner Chloroplast Membrane01:18

    Protein Transport to the Inner Chloroplast Membrane

    Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...