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Alteration of the faecal microbiota composition in patients with constipation: evidence of American Gut Project

Y S Xu1, Y H Wang2, Y Liu3

  • 1Department of Gastroenterology, The Affiliated Hospital of Qingdao University, Qingdao, China P.R.

Beneficial Microbes
|November 15, 2022
PubMed

Insights

Constipation patients exhibit reduced gut microbiota richness and evenness compared to healthy individuals. This gut dysbiosis, particularly in butyrate metabolism, may offer insights for clinical diagnosis and treatment.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Metagenomics

Background:

  • Limited understanding of gut microbiota composition differences in constipation.
  • Gut microbiota plays a crucial role in host health and gastrointestinal function.

Purpose of the Study:

  • To investigate the differences in gut microbiota composition between patients with constipation and healthy controls.
  • To explore the relationship between gut microbiota dysbiosis and butyrate metabolism in constipation.

Main Methods:

  • Analysis of faecal 16S rRNA sequencing data from the American Gut Project.
  • Application of propensity score matching (PSM) to control for confounding factors (age, gender, BMI, country).
  • Comparison of gut microbiota diversity, richness, evenness, and dominant genera between 262 constipation patients and 262 healthy controls.

Main Results:

  • Significantly lower gut microbiota richness and evenness observed in the constipation group.
  • Dominant genera in constipation: Escherichia_Shigella, Pseudomonas, Citrobacter. Dominant genera in controls: Faecalibacterium, Prevotella, Roseburia, Clostridium_XlVa, Blautia.
  • Increased abundance of three butyrate production-related pathways in the constipation group compared to controls.

Conclusions:

  • Constipation is associated with gut microbiota dysbiosis and altered butyrate metabolism.
  • This dysbiosis may serve as a potential biomarker for diagnosing and treating constipation-related conditions.
Keywords:
16SrRNAAmerican Gut Projectbutyrateconstipationmicrobiota

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