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Native DIGE for Quantitative and Functional Analysis of Protein Interactomes.

Diksha Dani1,2, Norbert A Dencher3

  • 1Institut für Biochemie und Biologie, Universität Potsdam, Potsdam-Golm, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2022
PubMed
Summary

This study introduces Blue Native Difference Gel Electrophoresis, a novel method to quantify changes in protein abundance and activity within protein complexes. It is particularly useful for studying membrane proteins and their interactions.

Keywords:
DIGENative PAGEPolyacrylaKey wordsmide gel electrophoresisProtein–protein interactionsProteomics

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