Analysis of the Saliva Proteome Using 2D-DIGE
Paul Dowling1, Eleanor M O'Sullivan2
1Department of Biology, Maynooth University, National University of Ireland, Maynooth, Co. Kildare, Ireland. paul.dowling@mu.ie.
Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2022
Summary
Saliva is a valuable biofluid containing biomolecules reflecting body
Area of Science:
- Biochemistry
- Molecular Biology
- Diagnostics
Background:
- Saliva is recognized as a crucial biofluid in research and clinical applications.
- It harbors diverse biomolecules such as proteins, metabolites, RNA, DNA, and microorganisms.
- These salivary components originate from blood, mirroring the body's physiological status.
Purpose of the Study:
- To highlight the significance of saliva as a diagnostic fluid.
- To underscore the role of omics approaches in salivary analysis.
- To emphasize the diagnostic potential of saliva for both oral and systemic diseases.
Main Methods:
- Utilizing advanced omics approaches for biomolecule identification and classification.
- Exploring various saliva collection methods and devices.
- Analyzing the composition of saliva for diagnostic markers.
Main Results:
- Saliva composition reflects systemic physiological conditions.
- Advanced omics techniques enable comprehensive salivary analysis.
- Saliva analysis facilitates the identification of biomarkers for diseases.
Conclusions:
- Saliva is a credible and non-invasive fluid for diagnosing oral and systemic diseases.
- The continuous advancement in omics technologies enhances saliva's diagnostic utility.
- Saliva analysis offers a promising avenue for personalized medicine and disease monitoring.
More Related Videos
14:48Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
7.1K
10:51Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
Published on: April 10, 2014
16.2K
Related Concept Videos
01:27SDS-PAGE
28.7K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
28.7K
01:22Two-dimensional Gel Electrophoresis
6.2K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.2K