Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

The Ratio of X Chromosome to Autosomes

The Ratio of X Chromosome to Autosomes

In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dual-mode visible/UV-blind Sb<sub>2</sub>Se<sub>3</sub> photodetector for secure single-pixel imaging.

Optics letters·2026

Same author

Rare-codon-tuned fluorescent biosensor for high-throughput selection of Escherichia coli strain capable of L-arginine overproduction.

BMC microbiology·2026

Same author

Vitamin D receptor isoform VDRA suppresses hepatocellular tumorigenesis by restricting YAP nuclear localization.

Cell death & disease·2026

Same author

<i>Anemarrhena asphodeloides</i> Polysaccharide Exerts Antiviral Activity Against Porcine Epidemic Diarrhea Virus Targeting ROS/Caspase-3 Dependent Apoptosis.

Transboundary and emerging diseases·2026

Same author

GEfetch2R: fetching single-cell/bulk RNA-seq data from public repositories to R and benchmarking the subsequent format conversion tools.

GigaScience·2026

Same author

A sequence motif for DNA double-strand break and telomere healing during programmed DNA elimination.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Aug 21, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

The nematode Oscheius tipulae as a genetic model for programmed DNA elimination.

Thomas C Dockendorff¹, Brandon Estrem¹, Jordan Reed¹

¹Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA.

Current Biology : CB

|November 15, 2022

Summary

Programmed DNA elimination (PDE) in Oscheius tipulae involves losing DNA during embryogenesis. A conserved Sequence For Elimination (SFE) motif is crucial for this process, establishing a new model for studying metazoan PDE.

Keywords:

CRISPR DNA double-strand break END-seq Oscheius tipulae genetic model nematode programmed DNA elimination sequence for elimination telomere addition wild isolates

More Related Videos

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

Related Experiment Videos

Last Updated: Aug 21, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Published on: November 14, 2016

Area of Science:

Genetics
Developmental Biology
Molecular Biology

Background:

Programmed DNA elimination (PDE) challenges genome integrity, often occurring during germline to somatic cell differentiation in metazoa.
Prior research described DNA sequences and chromosome behavior during metazoan PDE, but a functional model was lacking.

Purpose of the Study:

To establish a functional and genetic model for studying the mechanisms and consequences of PDE in the nematode Oscheius tipulae.
To investigate the molecular basis of DNA elimination in O. tipulae.

Main Methods:

Utilized staged embryos and DNA fluorescence in situ hybridization (FISH) to observe PDE timing.
Identified conserved Sequence For Elimination (SFE) motifs at DNA break sites.
Employed END-seq, SFE mutant genome sequencing, and comparative genomics to analyze elimination mechanisms and identify functional SFEs.

Main Results:

Demonstrated that O. tipulae PDE occurs during embryogenesis at the 8-16 cell stages.
Identified a conserved SFE motif at all 12 DNA break sites, with mutations causing a "fail-to-eliminate" phenotype.
END-seq revealed breaks within SFEs, extensive end resection, and telomere addition; numerous functional SFEs were identified at chromosome ends.

Conclusions:

Oscheius tipulae provides a new, tractable model for metazoan PDE research.
Alternative SFEs offer flexibility in DNA elimination and act as a fail-safe mechanism.
The conserved SFE motif is critical for precise DNA breakage and elimination during development.