Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same authorSame journal

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026

Same authorSame journal

Cryo-EM-guided subtractive optimization of a novel VCP/p97 inhibitor.

IUCrJ·2026

Same author

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Acta crystallographica. Section D, Structural biology·2026

Same author

Cryo-EM: the revolution continues.

IUCrJ·2026

Same author

Structural and functional mapping of protective human monoclonal antibodies against enterovirus A71.

Science advances·2026

Same author

Heterogeneous reconstruction algorithms for cryoEM achieve limited particle classification accuracy on real benchmark datasets.

bioRxiv : the preprint server for biology·2026

Same journal

Towards light-coupled sample preparation for time-resolved cryoEM studies.

IUCrJ·2026

Same journal

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026

Same journal

How cryoEM has advanced our understanding of bacteriophages and bacteriocins targeting Clostridioides difficile.

IUCrJ·2026

Same journal

CryoEM structures reveal allosteric regulation of the catalytic activity of the multi-protein human MAT enzyme complexes.

IUCrJ·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 21, 2025

Cryo-EM and Single-Particle Analysis with Scipion

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

Cryo-EM at ACA 2022.

Sriram Subramaniam^1,2, Abhay Kotecha³, Joseph H Davis⁴

¹Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada.

|November 16, 2022

Summary

This summary is machine-generated.

The 2022 American Crystallographic Association meeting highlighted structural biology advancements. There was a notable shift towards methods beyond traditional crystallography, particularly cryo-electron microscopy.

Area of Science:

Structural Biology
Biophysics
Biochemistry

Background:

The American Crystallographic Association (ACA) convenes annually to discuss structural biology.
The 2022 meeting in Portland addressed diverse topics in the field.

Keywords:

ACA 2022 cryo-EM editorial

More Related Videos

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Published on: December 1, 2023

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Published on: February 7, 2022

Related Experiment Videos

Last Updated: Aug 21, 2025

Cryo-EM and Single-Particle Analysis with Scipion

Cryo-EM and Single-Particle Analysis with Scipion

Published on: May 29, 2021

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Author Spotlight: Enhancing Cryo-Electron Microscopy by Automated Data Collection and Analysis Techniques

Published on: December 1, 2023

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Published on: February 7, 2022