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Related Concept Videos

X-ray Crystallography02:18

X-ray Crystallography

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The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
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Related Experiment Video

Updated: Aug 21, 2025

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
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Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.

Kara A Zielinski1, Andreas Prester2, Hina Andaleeb3

  • 1Center for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607 Hamburg, Germany.

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|November 16, 2022
PubMed
Summary

Serial synchrotron crystallography (SSX) now enables room-temperature data collection with minimal sample. A novel conveyor belt system significantly speeds up SSX, paving the way for high-throughput applications.

Keywords:
A. flavus urate oxidaseCFEL TapeDriveK. pneumoniae CTX-M-14N. haematococca GH11 xylanasepartial reflectionsprotein structuresroom temperaturesample deliveryserial crystallography

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Area of Science:

  • Structural biology
  • Biophysics
  • Biochemistry

Background:

  • Serial crystallography at synchrotron light sources (SSX) allows room-temperature data collection from micro-crystals of biological macromolecules.
  • Current SSX methods are less routine and slower than traditional cryo-crystallography, limiting high-throughput applications.
  • High-throughput SSX at physiological temperatures is desirable for fragment-based drug screening.

Purpose of the Study:

  • To develop a high-throughput SSX method using a conveyor belt sample delivery system.
  • To demonstrate the efficiency of the new system for collecting complete datasets from various proteins.

Main Methods:

  • Utilized the CFEL TapeDrive, a conveyor belt-based sample delivery system for SSX.
  • Tested the system with three proteins: Klebsiella pneumoniae CTX-M-14 β-lactamase, Nectria haematococca xylanase GH11, and Aspergillus flavus urate oxidase.
  • Collected complete diffraction datasets at room temperature.

Main Results:

  • Achieved complete dataset collection in under a minute per sample.
  • Required only minimal amounts of protein sample for data collection.
  • Successfully demonstrated the feasibility of high-throughput SSX with diverse biological proteins.

Conclusions:

  • The CFEL TapeDrive system significantly accelerates SSX data collection.
  • This advancement enables high-throughput SSX at room temperature with minimal sample consumption.
  • The developed method holds promise for applications like fragment-based drug screening.