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Related Experiment Video

Updated: Aug 21, 2025

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CKB affects human osteosarcoma progression by regulating the p53 pathway.

Fengxin Zhou1, Xinli Dou2, Chenguang Li1

  • 1Department of Orthopedics, Tianjin Integrative Medicine Hospital (Tianjin Nankai Hospital) Tianjin 300100, China.

American Journal of Cancer Research
|November 16, 2022
PubMed
Summary

The creatine kinase B (CKB) gene promotes human osteosarcoma (OS) development by impacting cell growth, migration, and apoptosis. CKB influences OS progression through the p53 signaling pathway.

Keywords:
CKBOsteosarcomacell invasioncell proliferationp53 signaling pathway

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma (OS) is a primary bone malignancy with significant mortality.
  • The role of creatine kinase B (CKB) in OS pathogenesis remains largely unexplored.

Purpose of the Study:

  • To investigate the functional role of the creatine kinase B (CKB) gene in human osteosarcoma (OS) development.
  • To elucidate the underlying molecular mechanisms by which CKB influences OS progression.

Main Methods:

  • Western blotting and qRT-PCR to quantify CKB expression in OS tissues and cells.
  • In vitro assays (CCK-8, colony formation, flow cytometry, Transwell, cell scratch) to assess OS cell viability, proliferation, apoptosis, invasion, and migration.
  • Gene Set Enrichment Analysis (GSEA) to identify affected signaling pathways.

Main Results:

  • CKB expression was significantly elevated in OS tissues and cells compared to normal controls.
  • CKB silencing inhibited OS cell proliferation, migration, and invasion while promoting apoptosis.
  • CKB overexpression enhanced OS cell proliferation, migration, and invasion while suppressing apoptosis.
  • CKB regulates the p53 signaling pathway, affecting the expression of key proteins like p53, p21, Bax, Bcl-2, and MDM2.
  • Modulation of p53 activity reversed the effects of CKB manipulation on OS cells.

Conclusions:

  • CKB plays a crucial role in promoting human osteosarcoma development.
  • CKB influences OS progression by regulating the p53 signaling pathway.
  • CKB represents a potential therapeutic target for osteosarcoma treatment.