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Pseudogene CSPG4P12 affects the biological behavior of non‑small cell lung cancer by Bcl‑2/Bax mitochondrial

Wenqian Hu1,2, Hongjiao Wu1, Ang Li1

  • 1School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063210, P.R. China.

Experimental and Therapeutic Medicine
|November 16, 2022
PubMed

Insights

Chondroitin sulfate proteoglycan 4 pseudogene 12 (CSPG4P12) is downregulated in non-small cell lung cancer (NSCLC). Overexpressing CSPG4P12 inhibits NSCLC progression by promoting apoptosis via the p53/Bcl2/Bax pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Chondroitin sulfate proteoglycan 4 (CSPG4) is implicated in tumor progression.
  • The specific role of chondroitin sulfate proteoglycan 4 pseudogene 12 (CSPG4P12) in non-small cell lung cancer (NSCLC) is not well understood.

Purpose of the Study:

  • To investigate the functional role of CSPG4P12 in NSCLC.
  • To elucidate the underlying molecular mechanisms by which CSPG4P12 affects NSCLC physiology.

Main Methods:

  • Analysis of CSPG4P12 expression in NSCLC tissues versus normal tissues using bioinformatics and RT-qPCR.
  • Assessment of cell proliferation, invasion, migration, and adhesion using various in vitro assays.
  • Detection of apoptosis and related protein expression (Bax, p53, Bcl2) via microscopy and Western blot.

Main Results:

  • CSPG4P12 expression was significantly lower in NSCLC tissues compared to adjacent normal tissues.
  • Overexpression of CSPG4P12 suppressed NSCLC cell proliferation, invasion, migration, and adhesion.
  • CSPG4P12 overexpression induced apoptosis, evidenced by morphological changes and altered expression of apoptosis-related proteins (increased Bax and p53, decreased Bcl2).

Conclusions:

  • CSPG4P12 acts as a tumor suppressor in NSCLC.
  • CSPG4P12 inhibits NSCLC development and tumorigenesis by activating the mitochondrial apoptotic pathway involving p53, Bcl2, and Bax.
Keywords:
chondroitin sulfate proteoglycan 4 pseudogene 12long non-coding RNAmitochondrial apoptosisnon-small cell lung cancerp53/Bcl2/Bax pathwaypseudogene

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