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Enhancing gene editing and gene targeting efficiencies in Arabidopsis thaliana by using an intron-containing version

Patrick Schindele1, Laura Merker1, Tom Schreiber2

  • 1Botanical Institute, Karlsruhe Institute of Technology, Karlsruhe, Germany.

Plant Biotechnology Journal
|November 16, 2022
PubMed
Abstract

No abstract available in PubMed .

Keywords:
CRISPR/Cas12agene editinggene targetingintronttLbCas12a

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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